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File:Phage display.png

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    Description
    English: Phage display cycle. 1) fusion proteins for a viral coat protein + the gene to be evolved (typically an antibody fragment) are expressed in bacteriophage. 2) the library of phage are washed over an immobilised target. 3) the remaining high-affinity binders are used to infect bacteria. 4) the genes encoding the high-affinity binders are isolated. 5) those genes may have random mutations introduced and used to perform another round of evolution. The selection and amplification steps can be performed multiple times at greater stringency to isolate higher-affinity binders.
    Français : traduction à améliorer : Cycle d'évolution. 1) Protéines de fusion pour une protéine virale d'enveloppe + le gène devant évoluer (typiquement un fragment d'anticorps) sont exprimées dans le bactériophage. 2) La banque de phages est lavées sur une cible immobilisée. 3) les liants à haute affinité restants sont utilisés pour infecter les bactéries. 4) les gènes codant pour les liants à haute affinité sont isolés. 5) ces gènes peuvent provoquer des mutations aléatoires introduites et utilisées pour effectuer un autre cycle d'évolution. Les étapes de sélection et d’amplification peuvent être effectuées plusieurs fois avec une plus grande rigueur pour isoler des liants d’affinité plus élevée.
    Date
    Source Own work
    Author Thomas Shafee

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    Captions

    Phage display cycle

    Items portrayed in this file

    depicts

    phage display

    directed evolution

    inception

    22 May 2019

    media type

    image/png

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    Date/TimeThumbnailDimensionsUserComment
    current07:53, 22 May 2019Thumbnail for version as of 07:53, 22 May 20192,826 × 1,798 (612 KB)Evolution and evolvabilityminor optimisation of label positions
    05:29, 22 May 2019Thumbnail for version as of 05:29, 22 May 20192,901 × 1,798 (585 KB)Evolution and evolvabilityUser created page with UploadWizard

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